Isolation and characterization of microsatellite markers for Dipteronia dyerana (Sapindaceae), an endangered endemic species in China

• Published in: American journal of botany Vol. 98; no. 10; pp. e271 - e273
• Main Authors: Chen, Chen, Ren, Bing‐Bing, Xu, Xi‐Hui, Fu, Cheng‐Xin, Qiu, Ying‐Xiong
• Format: Journal Article
• Language: English
• Published: United States Botanical Society of America 01.10.2011; Botanical Society of America, Inc
• Subjects: Alleles; Botany; China; Dipteronia dyerana; DNA, Plant - genetics; DNA, Plant - isolation & purification; Endangered & extinct species; Endangered Species; Flowers & plants; Genetic Loci - genetics; Genetic markers; Genetics, Population; microsatellite; Microsatellite Repeats - genetics; Molecular Sequence Data; polymorphic; Polymorphism; Sapindaceae; Sapindaceae - genetics; Species Specificity; China
• ISSN: 0002-9122; 1537-2197
• DOI: 10.3732/ajb.1100185

• Premise of the study: Microsatellite markers were developed for Dipteronia dyerana, an endangered endemic species in China, to study the population genetics of this species. • Methods and Results: Using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol, 12 microsatellite markers that were successfully amplified showed polymorphism when tested on 31 individuals from two populations in the counties of Wenshan and Mengzi. Overall, the number of alleles per locus ranged between seven and 25, with an average of 12.3. Nine of these markers were able to be amplified in D. sinensis. • Conclusions: These microsatellite markers should facilitate further studies on the population genetics and the reproductive ecology of Dipteronia.