Expression of CC Chemokine Ligand 5 in Patients with Rheumatoid Arthritis and Its Correlation with Disease Activity and Medication

• Published in: Chinese medical sciences journal Vol. 24; no. 1; pp. 50 - 54
• Main Authors: Yang, Ming-hui, Wu, Feng-xia, Xie, Chuan-mei, Qing, Yu-feng, Wang, Guang-rong, Guo, Xiao-lan, Tang, Zhong, Zhou, Jing-guo, Yuan, Guo-hua
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.03.2009; Institute of Rheumatology and Immunology, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, China
• Subjects: Adult; Aged; Arthritis, Rheumatoid - blood; Arthritis, Rheumatoid - drug therapy; Arthritis, Rheumatoid - metabolism; Arthritis, Rheumatoid - pathology; Blood Sedimentation; C-Reactive Protein - metabolism; CC chemokine ligand 5; chemokine; Chemokine CCL5 - analysis; Chemokine CCL5 - blood; Female; Humans; Joints - pathology; Male; Middle Aged; Osteoarthritis - blood; Osteoarthritis - metabolism; RA患者; rheumatoid arthritis; Synovial Fluid - metabolism; Young Adult; 关节炎患者; 疾病活动性; 类风湿关节炎; 药物治疗; 趋化因子配体; 酶联免疫吸附试验; 雷公藤多甙; CC chemokine ligand 5; rheumatoid arthritis; chemokine
• ISSN: 1001-9294
• DOI: 10.1016/S1001-9294(09)60059-6

Objective To determine the levels of CC chemokine ligand 5 （CCL5） in serum and synovial fluid （SF） from patients with rheumatoid arthritis （RA） and their relations with disease activity and medication. Methods CCL5 in serum and SF was quantified by enzyme-linked immunosorbent assay （ELISA） in 28 RA patients and 21 osteoarthritis （OA） patients. In RA patients, the correlations of CCL5 levels in serum and SF with disease activity were analyzed. Meanwhile, the serum CCL5 levels among RA patients treated with disease-modifying antirheumatic drugs （DMARDs）, Tripterygium Glucosides, and other Chinese herbs without disease-modifying effects were also compared. Results CCL5 levels in both serum and SF of RA patients were significantly higher than those of OA patients （P 〈 0.05）. Moreover, the level of CCL5 was higher in SF than that in serum of RA patients （P 〈 0.01）. Serum CCL5 level was correlated significantly with the number of swollen joints （r = 0.3329, P 〈 0.05）, erythrocyte sedimentation rate （r = 0.4001, P 〈 0.05）, and C reactive protein （r = 0.3735, P 〈 0.01） In addition, the level of CCL5 had a trend of lower in patients treated with DMARDs or Tripterygium Glucosides than those treated with other Chinese herbs, although the difference was not significant among those patients due to the small number of patients in each group. Conclusions In RA patients, the expression of CCL5 increases and correlates with some clinical and laboratory parameters of RA, which indicate that CCL5 plays an important role in RA and may serve as a useful marker of disease activity. DMARDs and Tripterygium Glucosides might exert their clinical effects through reducing CCL5 production in RA.