Chaperone-Like Functions of High-Mannose Type and Complex-Type N-Glycans and Their Molecular Basis
It has recently become apparent that high-mannose type N-glycans directly promote protein folding, whereas complex-type ones play a crucial role in the stabilization of protein functional conformations through hydrophobic interactions with the hydrophobic protein surfaces. Here an attempt was made t...
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Published in | Journal of biochemistry (Tokyo) Vol. 132; no. 5; pp. 803 - 811 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
England
Oxford University Press
01.11.2002
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Subjects | |
Online Access | Get full text |
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Summary: | It has recently become apparent that high-mannose type N-glycans directly promote protein folding, whereas complex-type ones play a crucial role in the stabilization of protein functional conformations through hydrophobic interactions with the hydrophobic protein surfaces. Here an attempt was made to understand more deeply the molecular basis of these chaperone-like functions with the aid of information obtained from spacefill models of N-glycans. The promotion of protein folding by high-mannose N-glycans seemed to be based on their unique structure, which includes a hydrophobic region similar to the cyclodextrin cavity. The promotive features of high-mannose N-glycans newly observed under various conditions furnished strong support for the view that both intra- and extramolecular high-mannose N-glycans are directly involved in the promotion of protein folding in the endoplasmic reticulum. Further, it was revealed that the N-acetyllactosamine units in complex-type N-glycans have an amphiphilic structure and greatly contribute to the formation of extensive hydrophobic surfaces and, consequently, to the N-glycan-protein hydrophobic interactions. The processing of high-mannose type N-glycans to complex-type ones seems to be an ingenious device to enable the N-glycans to perform these two chaperone-like functions. |
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Bibliography: | ArticleID:132.5.803 istex:02397DCF30A9B349C2AA770369F39EC0711976D7 1This study was supported in part by a Grant-in-Aid for Scientific Research (11480169) from the Ministry of Education, Science, Sports and Culture of Japan. 3To whom correspondence should be addressed at: 2-37, Minamikasugaoka 3-chome, Ibaraki, Osaka 567-0046. Tel: +81-726-25-8967, Fax: +81-726-25-8967, E-mail: simplife@hera.eonet.ne.jp ark:/67375/HXZ-BG7GJKCM-B ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0021-924X 1756-2651 |
DOI: | 10.1093/oxfordjournals.jbchem.a003290 |