Purification of small oligonucleotides by polyacrylamide gel electrophoresis and transfer to diethylaminoethyl paper

The application of polyacrylamide gel electrophoresis with subsequent electroelution onto DEAE paper for the purification of small oligonucleotides is described. We demonstrate that synthetic DNAs and hydrolyzed RNAs as small as three nucleotides in length can be purified by this technique. The prod...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 152; no. 2; pp. 221 - 225
Main Authors Vorndam, A.Vance, Kerschner, Joanne
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.02.1986
Subjects
DEAE
diethylaminoethyl
DNA
electroelution
Electrophoresis, Polyacrylamide Gel - methods
Ethanolamines
gel electrophoresis
low molecular weight
Oligodeoxyribonucleotides - isolation & purification
oligonucleotides
Oligonucleotides - isolation & purification
Oligoribonucleotides - isolation & purification
purification
RNA
DEAE
oligonucleotides
RNA
DNA
purification
electroelution
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Summary:The application of polyacrylamide gel electrophoresis with subsequent electroelution onto DEAE paper for the purification of small oligonucleotides is described. We demonstrate that synthetic DNAs and hydrolyzed RNAs as small as three nucleotides in length can be purified by this technique. The product is undegraded and homogeneous in length.
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ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(86)90401-X