Poly( o-aminophenol)-modified bienzyme carbon paste electrode for the detection of uric acid

• Published in: Talanta (Oxford) Vol. 43; no. 5; pp. 785 - 796
• Main Authors: Miland, E., Miranda Ordieres, A.J., Tuñón Blanco, P., Smyth, M.R., Fágáin, C.Ó.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.05.1996; Oxford Elsevier
• Subjects: Aminophenol(poly)-modified electrode; Biological and medical sciences; Biosensors; Biotechnology; Enzyme electrode; Fundamental and applied biological sciences. Psychology; Methods. Procedures. Technologies; Uric acid; Various methods and equipments; Aminophenol(poly)-modified electrode; Enzyme electrode; Uric acid; Performance evaluation; Human; Biological fluid; Enzyme; Urate oxidase; Coimmobilization; Carbon electrode; Optimization; Glow discharge polymerization; Peroxidases; Clinical biology; Amperometry; Serum; Paste electrode; Peroxidase; Oxidoreductases; Medical applications; Diagnosis; Biosensors; Immobilized enzyme; Quantitative analysis; Modified material
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/0039-9140(95)01825-5

A reagentless uric acid selective biosensor constructed by immobilising uricase and horseradish peroxidase (HRP) in carbon paste without the addition of an electron transfer mediator is described. The response of the electrode is based on the enzymatic reduction of hydrogen peroxide in the presence of uric acid. Uricase and HRP were dispersed in the carbon paste and the optimum paste mixture was determined. Poly( o-aminophenol) was electropolymerised at the working surface area of the electrode acting as a conducting polymer layer. Cyclic voltammetry was used to characterise the permselective characteristics of the polymer layer. At an applied potential of 50 mV vs. Ag/AgCl, a linear response was obtained up to 1 × 10 −4 M, with a limit of detection of 3 × 10 −6 M. The sensor had a response time of 37 s. a calibration precision of 2.2% ( n = 4) and an estimated sample frequency of 20 h −1. Responses to the analyte of interest were pH dependent. The sensor was incorporated into a flow injection system for the qualification of uric acid in human serum. Results compared favourably with a standard spectrophotometric method.