Characterization of a new stearoyl-acyl carrier protein desaturase gene from Jatropha curcas

• Published in: Biotechnology letters Vol. 28; no. 9; pp. 657 - 662
• Main Authors: LUO TONG, PENG, Shu-Ming, DENG, Wu-Yuan, MA, Dan-Wei, XU YING, XIAO MENG, CHEN FANG
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.05.2006; Springer Nature B.V
• Subjects: Acyl carrier protein; Amino Acid Sequence; Amino acids; Base Sequence; Biological and medical sciences; Biotechnology; Cloning, Molecular; Desaturase; DNA, Complementary - genetics; DNA, Plant - genetics; E coli; Escherichia coli - genetics; Ferredoxin; Fundamental and applied biological sciences. Psychology; Gene Expression; Genes; Genes, Plant; Jatropha - enzymology; Jatropha - genetics; Jatropha curcas; Mixed Function Oxygenases - genetics; Mixed Function Oxygenases - metabolism; Molecular Sequence Data; Nucleotide sequence; Proteins; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Seeds; Sequence Homology, Amino Acid; Characterization; Gene; fatty acid; prokaryotic expression; stearoyl-acyl carrier protein desaturase; Fatty acids; Carrier protein; Jatropha curcas
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-006-0034-3

A new full-length cDNA of stearoyl-acyl carrier protein desaturase was obtained by RT-PCR and RACE techniques from developing seeds of Jatropha curcas. Sequence alignment showed that its deduced amino acid sequence had high similarity with other stearoyl-acyl carrier protein desaturases. The gene was functionally expressed in E. coli and the desaturating activity of recombinant protein was easily detected when assayed in vitro with added spinach ferredoxin. Southern blot analysis indicated that the gene was a member of a small gene family. Northern blot analysis revealed it was highly expressed in developing fruits of J. curcas.