6-Benzylaminopurine Alleviates the Impact of Cu2+ Toxicity on Photosynthetic Performance of Ricinus communis L. Seedlings

• Published in: International journal of molecular sciences Vol. 22; no. 24; p. 13349
• Main Authors: Sameena, Puthukkolli P., Kalaji, Hazem M., Żuk-Gołaszewska, Krystyna, Horaczek, Tomasz, Sierka, Edyta, Puthur, Jos T.
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 12.12.2021; MDPI
• Subjects: 6-benzylaminopurine; Antioxidants; antioxidation; Benzyladenine; Bioaccumulation; Carotenoids; Chlorophyll; chlorophyll a fluorescence; cytokinin; Cytokinins; Efficiency; Electron transport; Fluorescence; Foliar applications; Heavy metals; Kinetin; Leaves; Lipid peroxidation; Lipids; membrane stability; Membranes; Metabolism; Metabolites; Parameters; Photosynthesis; Photosynthetic apparatus; Physiology; Pollutants; Ricinus communis; Seedlings; Spraying; Stomata; System effectiveness; Toxicity
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms222413349

Copper (Cu) is an essential element involved in various metabolic processes in plants, but at concentrations above the threshold level, it becomes a potential stress factor. The effects of two different cytokinins, kinetin (KIN) and 6-benzylaminopurine (BAP), on chlorophyll a fluorescence parameters, stomatal responses and antioxidation mechanisms in castor (Ricinus communis L.) under Cu2+ toxicity was investigated. Ricinus communis plants were exposed to 80 and 160 μM CuSO4 added to the growth medium. Foliar spraying of 15 μM KIN and BAP was carried out on these seedlings. The application of these cytokinins enhanced the tissue water status, chlorophyll contents, stomatal opening and photosynthetic efficiency in the castor plants subjected to Cu2+ stress. The fluorescence parameters, such as Fm, Fv/Fo, Sm, photochemical and non-photochemical quantum yields, energy absorbed, energy trapped and electron transport per cross-sections, were more efficiently modulated by BAP application than KIN under Cu2+ toxicity. There was also effective alleviation of reactive oxygen species by enzymatic and non-enzymatic antioxidation systems, reducing the membrane lipid peroxidation, which brought about a relative enhancement in the membrane stability index. Of the various treatments, 80 µM CuSO4 + BAP recorded the highest increase in photosynthetic efficiency compared to other cytokinin treatments. Therefore, it can be concluded that BAP could effectively alleviate the detrimental effects of Cu2+toxicity in cotyledonary leaves of R. communis by effectively modulating stomatal responses and antioxidation mechanisms, thereby enhancing the photosynthetic apparatus’ functioning.