Separation of anions by ion-interaction chromatography with a novel cationic/zwitterionic eluent

• Published in: Journal of Chromatography A Vol. 1020; no. 2; pp. 259 - 264
• Main Authors: Yan, Zhu, Yanyan, Ling, Fritz, James S, Haddad, Paul R
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 12.12.2003; Elsevier
• Subjects: Aerosol analysis; Aerosols; Analytical chemistry; Anions; Anions - isolation & purification; Cations; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, Liquid - methods; Conductivity detection; Exact sciences and technology; Hydrogen-Ion Concentration; Mobile-phase composition; Other chromatographic methods; Reproducibility of Results; Sensitivity and Specificity; Zwitterion; Zwitterion; Mobile-phase composition; Anions; Aerosol analysis; Conductivity detection; Chemical analysis; Phase composition; Nitrites; Ion pair; HPLC chromatography; Pyruvate; Nitrates; Mobile phase; Organic anion; Reversed phase chromatography; Inorganic anion; Conductometry; Halides; Aerosols; Quantitative analysis
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2003.08.009

Inorganic and organic anions can be separated on an ordinary silica C18 column using a mobile phase containing tetrabutylammonium hydroxide (TBAH) and an aminosulfonic acid zwitterion reagent (MOPS). The pH of this eluent is close to 7 and the background conductivity is about 50 μS, which is low enough to permit anion analyte detection by direct conductivity. Linear calibration curves were obtained for the six anions studied and detection limits ranged from 0.075 to 0.15 mg/l (ppm) for the five inorganic anions. The method was applied to the determination of water-soluble anions in aerosol samples at concentrations as low as 0.3 mg/l.