Electrophoretically mediated microanalysis

• Published in: Journal of Chromatography A Vol. 1032; no. 1; pp. 173 - 184
• Main Authors: Nováková, Soňa, Van Dyck, Sigrid, Van Schepdael, Ann, Hoogmartens, Jos, Glatz, Zdeněk
• Format: Book Review Conference Proceeding Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 02.04.2004; Elsevier
• Subjects: Analytical biochemistry: general aspects, technics, instrumentation; Analytical chemistry; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Chemistry; Chip technology; Chromatographic methods and physical methods associated with chromatography; Electrophoresis, Capillary - methods; Electrophoretically mediated microanalysis; Enzymes; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Other chromatographic methods; Reviews; Semiconductors; Enzymes; Chip technology; Reviews; Electrophoretically mediated microanalysis; Capillary electrophoresis; Enzyme; Microanalysis; System on a chip; Review; Microequipment; Miniaturization; Application; Microfluidics
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2003.12.025

This review describes the existing developments in the use of the capillary electrophoretic microanalytical technique for the in-line study of enzyme reaction, electrophoretically mediated microanalysis (EMMA). The article is divided into a number of parts. After an introduction, the different modes, basic principle, procedure, and some mathematical treatments of EMMA methodology are discussed and illustrated. The applications of EMMA for enzyme assay and for non-enzymatic determination are summarized into two tables. In addition to classical capillary electrophoresis (CE) instrument EMMA, special emphasis is given to a relatively new technique: EMMA on CE microchip. Finally, conclusions are drawn.