Simultaneous determination of Vitamin E homologs in chicken meat by liquid chromatography with fluorescence detection

• Published in: Journal of Chromatography A Vol. 1025; no. 2; pp. 313 - 317
• Main Authors: Hewavitharana, A.K., Lanari, M.C., Becu, C.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 06.02.2004; Elsevier
• Subjects: Animals; Biological and medical sciences; Chickens; Chromatography, Liquid - methods; Food analysis; Food industries; Fundamental and applied biological sciences. Psychology; Meat; Meat and meat product industries; Poultry Products - analysis; Spectrometry, Fluorescence - methods; Tocopherols; Tocotrienols; Vitamin E - analysis; Vitamins; Tocopherols; Food analysis; Tocotrienols; Vitamins; Meat; Fluorescence detector; Chemical analysis; Vitamins: Tocopherols; Liquid chromatography; E-Vitamins; Chicken meat; Simultaneous measurement; Homologous series; Qualitative analysis; Tocopherol; Quantitative analysis
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2003.10.052

A simple and reliable method for the simultaneous determination of all eight homologs of Vitamin E in chicken meat is described. All analytes, including the internal standard (α-tocopherol acetate), were eluted within 35 min and detected using their native fluorescence (295 nm excitation and 330 nm emission). Chromatography using hexane based eluent on a normal phase silica column included an initial column conditioning step to prevent irreversible adsorption of tocopherols and tocotrienols on silica. Lowest detectable levels of α-tocopherol, γ-tocopherol, α-tocotrienol, β-tocotrienol, γ-tocotrienol and δ-tocotrienol were 0.73, 0.86, 1.0, 1.2, 1.7 and 1.3 ng, respectively.