Molecular Analysis of Salmonella Enteritidis and Typhimurium Clinical and Food Isolates by Pulsed-Field Gel Electrophoresis in Bogotá, Colombia
In 1997, the Microbiology Group of the Colombian Instituto Nacional de Salud set up a surveillance program with the National Public Health Laboratories to monitor the principal etiological agents responsible for acute diarrheal disease. The main goal of this study was to determine the Xba I DNA dige...
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Published in | Microbial drug resistance (Larchmont, N.Y.) Vol. 12; no. 1; pp. 68 - 73 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
Mary Ann Liebert, Inc
01.03.2006
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Subjects | |
Online Access | Get full text |
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Summary: | In 1997, the Microbiology Group of the Colombian Instituto Nacional de Salud set up a surveillance program
with the National Public Health Laboratories to monitor the principal etiological agents responsible for acute
diarrheal disease. The main goal of this study was to determine the
Xba
I DNA digestion patterns of clinical
and food
Salmonella
spp. isolates recovered in Bogotá from 1997 to 2002, and related them to the susceptibility
patterns to antimicrobial agents. Two hundred and twenty-four
Salmonella
spp. isolates were studied,
153 (63%)
S
. Enteritidis and 71 (37%)
S
. Typhimurium. Pulsed-field gel electrophoresis (PFGE) was done using the
Xba
I restriction enzyme and
Salmonell
a Braenderup H9812 as the molecular weight marker. For
S
. Enteritidis, pattern S. En0001 was found to be prevalent in 127 (83%) isolates, 78 (61%) susceptible to the
antimicrobial agents tested. For S. Typhimurium, pattern S. Typ0001 was predominant in 18 (25%) isolates
with different antimicrobial resistance profiles. Patterns S. En0001 and S. Typ0001 prevailed monthly during
the 6 years of the study. Data collected demonstrate that there was a dominant pattern of S. Enteritidis
and S. Typhimurium circulating in clinical and food isolates in Bogotá, Colombia. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1076-6294 1931-8448 |
DOI: | 10.1089/mdr.2006.12.68 |