Development of a Tri-Functional Nanoprobe for Background-Free SERS Detection of Sialic Acid on the Cell Surface

Sialic acid (SA) on the surface of cells is indispensable in numerous physiological and pathological processes, and sensitive and reproducible detection of SA is crucial for diagnosis and therapy in many diseases. Here, we developed a tri-functional nanoprobe as a sensitive and straightforward surfa...

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Bibliographic Details
Published inChemosensors Vol. 9; no. 5; p. 92
Main Authors Renata, Septila, Verma, Nitish, Tu, Zhijay, Pan, Rong-Long, Hofmann, Mario, Lin, Chun-Hung
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.05.2021
Subjects
Ammonium
Binding sites
biosensor
Cancer
Cell surface
Chromatography
cyano
Metastasis
Nanoparticles
NMR
Nuclear magnetic resonance
phenylboronic acid
Raman
Raman spectroscopy
Reagents
Recognition
Scanning electron microscopy
sialic acid
Spatial discrimination
Spatial resolution
Spectrum analysis
surface-enhanced Raman spectroscopy (SERS)
United Kingdom > UK
United States > US
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Summary:Sialic acid (SA) on the surface of cells is indispensable in numerous physiological and pathological processes, and sensitive and reproducible detection of SA is crucial for diagnosis and therapy in many diseases. Here, we developed a tri-functional nanoprobe as a sensitive and straightforward surface-enhanced Raman spectroscopy (SERS) nanoprobe for sialoglycan detection on cell surfaces. The reporter was designed to provide three key functionalities that make it ideal for SA detection. First, we employed two recognition groups, phenylboronic acid and an ammonium group, that enhance SA recognition and capture efficiency. Second, we used cyano as the Raman reporter because it emits in the cellular Raman silent region. Finally, thiol acted as an anchoring agent to conjugate the reporter to silver nanocubes to provide SERS enhancement. Our molecular nanoprobe design demonstrated the ability to detect SA on the cell surface with high sensitivity and spatial resolution, opening up new routes to cellular diagnostics.
ISSN:2227-9040
2227-9040
DOI:10.3390/chemosensors9050092