Nucleotide sequence of 4.5S RNA (C8 or hY5) from HeLa cells

The nucleotide sequence of C8 RNA, one of the 4.5S RNAs of HeLa cells, was determined. C8 RNA consists of 83 or 84 nucleotide residues containing pppA at its 5′-terminus and oligo U at its 3′-terminus. This RNA is rich in uridylate residues (about 38%) and does not have any modified nucleosides. C8...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 108; no. 1; pp. 363 - 370
Main Authors Kato, Nobuyuki, Hoshino, Hiro-o, Harada, Fumio
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 16.09.1982
Subjects
Base Sequence
Cell Nucleus - analysis
Endoribonucleases
HeLa Cells - analysis
Humans
Molecular Weight
Nucleic Acid Conformation
Ribonuclease T1
Ribonuclease, Pancreatic
RNA, Neoplasm - isolation & purification
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Summary:The nucleotide sequence of C8 RNA, one of the 4.5S RNAs of HeLa cells, was determined. C8 RNA consists of 83 or 84 nucleotide residues containing pppA at its 5′-terminus and oligo U at its 3′-terminus. This RNA is rich in uridylate residues (about 38%) and does not have any modified nucleosides. C8 RNA is present mainly in the cytoplasm, with some in the nucleus and the C8 RNAs from the nucleus and cytoplasm have the same sequence.
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ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(82)91875-7