Janibacter Species with Evidence of Genomic Polymorphism Isolated from Resected Heart Valve in a Patient with Aortic Stenosis

The authors report isolation and identification of two strains of bacteria belonging to the genus Janibacter from a human patient with aortic stenosis from a rural area of the country of Georgia. The microorganisms were isolated from aortic heart valve. Two isolates with slightly distinct colony mor...

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Bibliographic Details
Published inInfectious disease reports Vol. 11; no. 2; p. 8132
Main Authors Malania, Lile, Bai, Ying, Khanipov, Kamil, Tsereteli, Marika, Metreveli, Mikheil, Tsereteli, David, Sidamonidze, Ketevan, Imnadze, Paata, Fofanov, Yuriy, Kosoy, Michael
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 18.09.2019
PAGEPress Publications, Pavia, Italy
Subjects
Aorta
Aortic stenosis
Bacteria
Gene sequencing
genome polymorphism
Genomes
Heart valves
Janibacter
Microorganisms
Morphology
Polymorphism
Rural areas
Stenosis
Strains (organisms)
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Summary:The authors report isolation and identification of two strains of bacteria belonging to the genus Janibacter from a human patient with aortic stenosis from a rural area of the country of Georgia. The microorganisms were isolated from aortic heart valve. Two isolates with slightly distinct colony morphologies were harvested after sub-culturing from an original agar plate. Preliminary identification of the isolates is based on amplification and sequencing of a fragment of 16SrRNA. Whole genome sequencing was performed using the Illumina MiSeq instrument. Both isolates were identified as undistinguished strains of the genus Janibacter. Characterization of whole genome sequences of each culture has revealed a 15% difference in gene profile between the cultures and confirmed that both strains belong to the genus Janibacter with the closest match to J. terrae. Genomic comparison of cultures of Janibacter obtained from human cases and from environmental sources presents a promising direction for evaluating a role of these bacteria as human pathogens.
Bibliography:Contributions: LM, YB, and KK contributed equally. LM, MK, conception and design; LM, MT, MM, DT, data collecting; LM, KS, bacteriological analysis; YB, KS, sequence analyses; KH, YF, genome analysis; LM, YB, MK, manuscript writing; MK, PI, manuscript reviewing and final approval.
Conflict of interests: the authors declare no potential conflict of interests.
ISSN:2036-7449
2036-7430
2036-7449
DOI:10.4081/idr.2019.8132