Analysis of IgE binding proteins of mesquite ( Prosopis juliflora) pollen and cross-reactivity with predominant tree pollens

• Published in: Immunobiology (1979) Vol. 211; no. 9; pp. 733 - 740
• Main Authors: Dhyani, Anamika, Arora, Naveen, Gaur, Shailendra N., Jain, Vikram K., Sridhara, Susheela, Singh, Bhanu P.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier GmbH 01.01.2006
• Subjects: Adolescent; Adult; Ailanthus; Ailanthus - immunology; Allergens; Antigens, Plant - chemistry; Antigens, Plant - immunology; Blotting, Western; Cassia siamea; Characterization; Cinnamomum aromaticum - immunology; Cross Reactions; Cross-reactivity; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Humans; Hypersensitivity, Immediate - diagnosis; Immunoglobulin E - blood; Middle Aged; Plant Extracts - chemistry; Plant Extracts - immunology; Pollen - chemistry; Pollen - immunology; Prosopis; Prosopis - chemistry; Prosopis - immunology; Prosopis juliflora; Salvadora persica; Salvadoraceae - immunology; Skin Tests; Tree pollen; Ulmus - immunology; Characterization; Tree pollen; Allergens; Prosopis juliflora; Cross-reactivity
• ISSN: 0171-2985; 1878-3279
• DOI: 10.1016/j.imbio.2006.03.003

Pollen from the mesquite tree, Prosopis juliflora, is an important source of respiratory allergy in tropical countries. Our aim was to partially characterize the IgE binding proteins of P. juliflora pollen extract and study cross-reactivity with prevalent tree pollen allergens. Intradermal tests with P. juliflora and five other tree pollen extracts were performed on respiratory allergy patients from Bikaner (arid) and Delhi (semi arid). Prosopis extract elicited positive skin reactions in 71/220 of the patients. Sera were collected from 38 of these 71 patients and all demonstrated elevated specific IgE to P. juliflora. Immunoblotting with pooled patients’ sera demonstrated 16 IgE binding components, with components of 24, 26, 29, 31, 35, 52, 58, 66 and 95 kDa recognized by more than 80% of individual patients’ sera. P. juliflora extract is allergenically potent requiring 73 ng of self-protein for 50% inhibition of IgE binding in ELISA inhibition. Cross-inhibition assays showed close relationship among P. juliflora, Ailanthus excelsa, Cassia siamea and Salvadora persica. IgE binding components of 14, 41, 52 and 66 kDa were shared allergens whereas 26 and 29 kDa were specific to P. juliflora. The findings suggest that purification of cross-reactive allergens will be helpful for diagnosis and immunotherapy of tree pollen allergic patients.