Associations of the SCD1 gene SNP with fatty acids composition of Holstein cows

• Published in: BIO Web of Conferences Vol. 27; p. 60
• Main Authors: Safina, Natalia Yu, Shakirov, Shamil K., Ravilov, Rustam Kh, Sharafutdinov, Gazimzyan S.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Les Ulis EDP Sciences 01.01.2020
• Subjects: Agricultural production; Animal fat; Animals; Composition; Dairy cattle; Deoxyribonucleic acid; Desaturation; DNA; DNA testing; Farms; Fatty acid composition; Fatty acids; Gene polymorphism; Genotype & phenotype; Genotypes; Milk; Polymerase chain reaction; Polymorphism; Restriction fragment length polymorphism; Scd1 protein; Single-nucleotide polymorphism
• ISSN: 2117-4458; 2273-1709; 2117-4458
• DOI: 10.1051/bioconf/20202700060

The research presents the findings of DNA testing of allelic polymorphism by the PCR-RFLP method of the SCD1 gene and the relationship of genotypes with the milk fatty acid composition of Holstein cows of Tatarstan population. The research was conducted among 172 Holstein cows at the Integrated Agricultural Production Centre “Stud farm named after Lenin” in Atninsky district of the Republic of Tatarstan in 2017–2018. All genotypes of the SCD1 gene were identified through the research. The milk yield test showed that the maximum content of the mass fraction of fat was in animals with the TT genotype. The significant difference between the content of the mass fraction of fat in milk in individuals by the locus of the SCD1 -Fsp4H I gene was: TT to CC – 0.32 % (p < 0.05) and TT to TC – 0.40 % (p < 0.01). The results of chromatography indicate the balance of the milk fatty acid composition of cows with the TT genotype and, as a consequence, its positive effect on the human body. Cows with a homozygous TT genotype are characterized by the optimum ratio of fatty acids in milk fat. The maximum value of the oxygen desaturation index is also observed in animals with the TT genotype by the SCD1 gene.