Proteome analysis of Citrus sinensis L. (Osbeck) flesh at ripening time

• Published in: Journal of proteomics Vol. 73; no. 1; pp. 134 - 152
• Main Authors: Muccilli, Vera, Licciardello, Concetta, Fontanini, Debora, Russo, Maria Patrizia, Cunsolo, Vincenzo, Saletti, Rosaria, Reforgiato Recupero, Giuseppe, Foti, Salvatore
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 02.11.2009; Elsevier
• Subjects: Anthocyanin; Biological and medical sciences; Citrus sinensis - metabolism; Diverse techniques; Fruit - metabolism; Fruit flesh; Fundamental and applied biological sciences. Psychology; LC-MS/MS; Models, Biological; Molecular and cellular biology; Proteome - analysis; Proteome - isolation & purification; Proteomics - methods; Ripening; Species Specificity; Sweet orange; Time Factors; Anthocyanin; Ripening; LC-MS/MS; Sweet orange; Fruit flesh; Citrus sinensis; Fruit; Rutaceae; Proteome; Time; Dicotyledones; Analysis; Angiospermae; Spermatophyta
• ISSN: 1874-3919; 1876-7737
• DOI: 10.1016/j.jprot.2009.09.005

A combination of 2-DE and LC-MSMS approaches was used to identify the differentially expressed proteome of a pigmented sweet orange ( Citrus sinensis, cv. Moro) in comparison with a common cultivar (Cadenera) at ripening time. The comparison of the protein patterns of Moro and Cadenera showed 64 differential expressed protein spots. Fifty-five differentially expressed proteins were identified. Proteins were classified according to their putative function and known biosynthetic pathways. Most of the proteins related to sugar metabolism were overexpressed in Moro, while those related to stress responses were overexpressed in Cadenera. The abundance of proteins belonging to Unknown/Unnamed and Hypothetical classes could be associated to the incomplete data available on the Citrus genome. The relative abundance of Secondary metabolism and Oxidative process proteins substantiated the key role of the anthocyanin pathway in Moro, which is characterized by a strong pigmentation at ripening time. The potential role of protein differential expression between Moro and Cadenera fruits was discussed, and proteomic results were compared with the known variations of transcripts of the same fruits. The latter analyses highlighted many discrepancies, confirming the necessity to associate both proteomic and transcriptomic approaches in order to achieve a more complete characterization of the biological system. 2-DE and LC-MSMS were used to identify 55 differentially expressed proteins in pigmented and common oranges. Proteins were classified and discussed according to their function and known biosynthetic pathways.