Immunoperoxidase staining for the detection of autoantibodies in canine autoimmune skin disease; Comparison to immunofluorescence results

Skin sections from 22 dogs with autoimmune skin disease were stained with anti-canine IgG, IgM and IgA using an immunobridge immunoperoxidase method. Eight cases of lupus erythematosus, three cases of pemphigus vulgaris, and 11 cases of pemphigus foliaceus were included. Results of previously perfor...

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Bibliographic Details
Published inVeterinary immunology and immunopathology Vol. 26; no. 2; pp. 105 - 113
Main Authors Bradley, Gregory A., Calderwood Mays, M.B.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.10.1990
Subjects
Animals
Autoantibodies - analysis
Autoimmune Diseases - diagnosis
Autoimmune Diseases - immunology
Autoimmune Diseases - veterinary
Dog Diseases - diagnosis
Dog Diseases - immunology
Dogs
Evaluation Studies as Topic
Fluorescent Antibody Technique - veterinary
Immunoenzyme Techniques - veterinary
Lupus Erythematosus, Cutaneous - immunology
Lupus Erythematosus, Cutaneous - veterinary
Pemphigus - immunology
Pemphigus - veterinary
Retrospective Studies
Skin Diseases - diagnosis
Skin Diseases - immunology
Skin Diseases - veterinary
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Summary:Skin sections from 22 dogs with autoimmune skin disease were stained with anti-canine IgG, IgM and IgA using an immunobridge immunoperoxidase method. Eight cases of lupus erythematosus, three cases of pemphigus vulgaris, and 11 cases of pemphigus foliaceus were included. Results of previously performed, direct immunofluorescence tests for the detection of canine immunoglobulin on skin were available on 17 22 cases. The immunoperoxidase method yielded an overall positive result in 59% ( 5 8 lupus erythematosus, 2 3 pemphigus vulgaris and 6 11 pemphigus foliaceus) versus an overall positive result of 47% for direct immunofluorescence ( 3 5 lupus erythematosus, 2 2 pemphigus vulgaris and 2 10 pemphigus foliaceus). The immunobridge immunoperoxidase method compared favorably to direct immunofluorescence testing of canine skin for autoantibody in cases of lupus erythematosis and pemphigus vulgaris, and was superior in cases of pemphigus foliaceus. This method should prove useful as an aid in the diagnosis of canine autoimmune skin disease.
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ISSN:0165-2427
1873-2534
DOI:10.1016/0165-2427(90)90060-6