Efficacy of trimming chilled beef during fabrication to control Escherichia coli O157:H7 surrogates on subsequent subprimals

• Published in: Meat science Vol. 90; no. 2; pp. 420 - 425
• Main Authors: Laster, B.A., Harris, K.B., Lucia, L.M., Castillo, A., Savell, J.W.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.02.2012
• Subjects: Animals; Beef; Cattle; Colony Count, Microbial; Escherichia coli O157; Escherichia coli O157 - isolation & purification; Escherichia coli O157 - pathogenicity; Escherichia coli O157:H7; Fabrication; Food Contamination - prevention & control; Food Handling - methods; Food Microbiology; gelatin; Meat - microbiology; pathogens; slurries; Subprimals; surface area; Trimming; Fabrication; Beef; Subprimals; Escherichia coli O157:H7; Trimming
• ISSN: 0309-1740; 1873-4138
• DOI: 10.1016/j.meatsci.2011.08.011

Effectiveness of trimming external carcass surfaces from subprimals during fabrication to reduce Escherichia coli O157:H7 surrogates was evaluated. Carcass sides ( n = 10 sides) were inoculated along the hide pattern opening before entering the blast chill cooler with a gelatin slurry containing a bacterial cocktail of three rifampicin-resistant, nonpathogenic E. coli biotype I strains. Following a 48 h chill, sides were fabricated to produce eight subprimals. Microbiological samples were taken from the original carcass fat surface area, initial lean surface area, trimmed fat surface area (where applicable), and trimmed lean surface area (where applicable). Newly exposed lean surfaces had lower ( P < 0.05) counts of rifampicin-resistant E. coli than did the external fat surfaces. However, fat and lean surfaces that were not inoculated became contaminated during the fabrication process. Trimming external surfaces reduced levels of pathogens, but under normal fabrication processes, pathogens were still spread to newly exposed surfaces. ► Evaluated trimming of chilled subprimal surfaces to reduce surrogate bacteria. ► Inoculated the brisket, plate, flank, and inside round with surrogate bacteria. ► Uninoculated fat and lean surfaces became contaminated during fabrication. ► Trimming subprimals surfaces reduced levels of surrogate bacteria. ► Bacteria were spread to newly exposed fat and lean surfaces during fabrication.