Oocyte maturation with royal jelly increases embryo development and reduces apoptosis in goats
Royal jelly (RJ) was supplemented to goat oocyte in vitro maturation (IVM) medium at three different concentrations (2.5, 5, and 10 mg/ml). Maturation rate, embryo cleavage, and blastocyst rate were recorded. Gene expression of apoptosis-related transcripts was investigated in matured oocytes. Perce...
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Published in | Animal reproduction Vol. 15; no. 2; pp. 124 - 134 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Colégio Brasileiro de Reprodução Animal
16.08.2018
|
Subjects | |
Online Access | Get full text |
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Summary: | Royal jelly (RJ) was supplemented to goat oocyte
in vitro
maturation (IVM)
medium at three different concentrations (2.5, 5, and 10 mg/ml). Maturation rate, embryo
cleavage, and blastocyst rate were recorded. Gene expression of apoptosis-related transcripts
was investigated in matured oocytes. Percentage of oocytes that reached MII-stage was increased
in RJ-treated groups compared to the control group. Glutathione (GSH) content of mature oocytes
was enhanced when RJ was added to IVM medium at any supplementation compared with control.
Percentage of cleaved embryos and blastocysts was higher in the RJ-treated groups at a concentration
of 5 than in the 2.5 mg/ml and control group. Total number of cells per blastocyst was not different
in the control and RJ-treated group at 5 mg/ml. However, number of apoptotic cells per blastocyst
was higher in the control group than in the RJ-treated group at 5 mg/ml. Expression profile
of
Bax
, and
p53
was down-regulated while
Bcl-2
was up-regulated in oocytes treated with RJ at 5 and 10 mg/ml compared with
the control group. Addition of RJ at concentrations of 5 mg/ml improved embryo production
through increasing maturation rate. RJ seems to improve the IVM microenvironment by reducing
expression of genes inducing apoptosis, enhancing GSH content, and reducing incidence of
apoptosis in blastocysts. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1984-3143 1806-9614 1984-3143 |
DOI: | 10.21451/1984-3143-2017-AR986 |