Free radical scavenging activity of type II collagen peptides and chondroitin sulfate oligosaccharides from by-products of mottled skate processing

• Published in: Food bioscience Vol. 41; p. 100991
• Main Authors: Li, Wen, Kobayashi, Taishi, Meng, DaWei, Miyamoto, Nobuyuki, Tsutsumi, Naonobu, Ura, Kazuhiro, Takagi, Yasuaki
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.06.2021
• Subjects: Antioxidant activity; Chondroitin sulfate oligosaccharides; Non-enzymatic browning products; Raja pulchra; Skate by-product; Type II collagen peptides; DPPH; Antioxidant activity; Non-enzymatic browning products; Raja pulchra; CP; CS; Chondroitin sulfate oligosaccharides; SDS-PAGE; Skate by-product; Ch-CP; Type II collagen peptides; Pa-CP; ABTS; CSo; and Tr-CP
• ISSN: 2212-4292; 2212-4306
• DOI: 10.1016/j.fbio.2021.100991

The by-products of the skate processing industry have bioactive properties but are mostly discarded. In the present study, the properties of skate cartilage, a major by-product of skate processing that is rich in chondroitin sulfate (CS) and type II collagen were investigated. Enzymatically-hydrolyzed type II collagen peptides (CP) and thermally hydrolyzed CS oligosaccharides (CSo) from skate cartilage were prepared and their antioxidant activities were studied. High free radical scavenging activity was observed in the <3 kDa MW fraction of the papain hydrolyzed CP (Pa-CP). CSo also had high free radical scavenging activity, but the activity was mainly due to the intermediate and final products of the non-enzymatic browning reaction resulting from high-temperature hydrolysis. These products may have included flavonoid-like compounds. Furthermore, Pa-CP and CSo were not cytotoxic. Thus, Pa-CP and CSo derived from skate by-products may be beneficial antioxidant ingredients. The present study may contribute to the development of functional health foods and achieving zero-waste skate processing. [Display omitted]