Biochemical Evaluation of Oxidative Stress during Exercise in Patients with Coronary Heart Disease

• Published in: Clinical chemistry and laboratory medicine Vol. 39; no. 3; pp. 234 - 238
• Main Authors: Andican, Gülnur, Koldaş, Lale, Seven, Arzu, Ayan, Faruk, Sirmaci, Necati, Burçak, Gülden
• Format: Journal Article
• Language: English
• Published: Berlin Walter de Gruyter 01.03.2001; New York, NY
• Subjects: Adult; Aged; Antioxidants - analysis; Biological and medical sciences; Biomarkers - blood; Cardiology. Vascular system; Cardiovascular system; Coronary Disease - physiopathology; Coronary heart disease; Exercise; Female; Glutathione - blood; Glutathione Peroxidase - blood; Heart; Humans; Investigative techniques, diagnostic techniques (general aspects); Male; Medical sciences; Middle Aged; Oxidative Stress; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Superoxide Dismutase - blood; Thiobarbituric Acid Reactive Substances - analysis; Vitamin E - blood; Physical exercise; Glutathione peroxidase; Human; Oxidative stress; Biochemical analysis; Enzyme; Cardiovascular disease; Exploration; Superoxide dismutase; E-Vitamins; Antioxidant; Coronary heart disease; Peroxidases; Enzymatic activity; Exercise tolerance test; Oxidoreductases; Quantitative analysis; Glutathione
• ISSN: 1434-6621; 1437-4331
• DOI: 10.1515/CCLM.2001.037

The impact of exercise tolerance test on oxidative stress was assessed by thiobarbituric acid reactive substances and markers of antioxidant status, namely Cu Zn superoxide dismutase, glutathione peroxidase, glutathione and vitamin E in blood samples of patients with exertional angina. The study was aimed to differentiate patients with positive exercise test (coronary heart disease patients) from patients with negative exercise test, at rest and peak exercise with respect to the investigated variables. Significantly lower values for both glutathione peroxidase activity and glutathione level were observed in patients after exercise test (p﹤0.01 and p﹤0.05, respectively). Only the patients with positive exercise test had significantly lower values for Cu Zn superoxide dismutase, glutathione peroxidase and glutathione, and a significantly higher ratio of thiobarbituric acid reactive substances/glutathione after exercise, as compared to before (p﹤0.05, p﹤0.05, p﹤0.05, p﹤0.01, respectively). Our findings indicate that the exercise test applied to patients with exertional angina oxidatively stresses the erythrocytes to a greater extent in exercise test (+) patients than in exercise test (−) patients.