Simultaneous determination of ergosterol, nucleosides and their bases from natural and cultured Cordyceps by pressurised liquid extraction and high-performance liquid chromatography

• Published in: Journal of Chromatography A Vol. 1036; no. 2; pp. 239 - 243
• Main Authors: Li, S.P, Li, P, Lai, C.M, Gong, Y.X, Kan, Kelvin K.W, Dong, Tina T.X, Tsim, Karl W.K, Wang, Y.T
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 21.05.2004; Elsevier
• Subjects: Analysis; Analytical biochemistry: general aspects, technics, instrumentation; Analytical chemistry; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid - methods; Cordyceps - chemistry; Cordyceps spp; Ergosterol; Ergosterol - analysis; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; General pharmacology; Medical sciences; Nucleosides; Nucleosides - analysis; Pharmaceutical analysis; Pharmacology. Drug treatments; Spectrophotometry, Ultraviolet; Sterols; Nucleosides; Ergosterol; Pharmaceutical analysis; Sterols; Cordyceps spp; Steroid; HPLC chromatography; Solvent extraction; Operating conditions; Pressure effect; Sample preparation; Analysis; Simultaneous measurement; Nucleoside; Sterol
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2004.02.080

A simple method is described for the simultaneous determination of ergosterol, nucleosides and their bases in Cordyceps. The samples were extracted by using pressurised liquid extraction (PLE). The effects of experimental variables, such as solvent, temperature, static extraction time and cycles, on PLE efficiency have been studied. The results showed a strong influence of the solvent and temperature on extraction efficiency of PLE. The determination was achieved by high-performance liquid chromatography (HPLC) using a Zorbax NH 2 analytical column (250×4.6 mm i.d., 5 μm) with diode-array detector (DAD). The automated preparation of the sample permits a very fast analysis which is an important goal for routine purpose.