Changes in the expression of fc receptor produced by induction of Epstein-Barr virus in lymphoma cell lines

• Published in: Virology (New York, N.Y.) Vol. 120; no. 2; pp. 376 - 382
• Main Authors: Yee, C., Costa, J., Hamilton, V., Klein, G., Rabson, A.S.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 30.07.1982
• Subjects: Animals; Antigens, Viral - biosynthesis; Butyrates - pharmacology; Callitrichinae; Cell Line; cell lines; Cell Transformation, Viral; Epstein-Barr virus; Flow Cytometry; Herpesvirus 4, Human - growth & development; Humans; lymphoma; Lymphoma - metabolism; man; Neoplasms, Experimental; receptors; Receptors, Fc - biosynthesis; Tetradecanoylphorbol Acetate - pharmacology; Virus Activation - drug effects
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1016/0042-6822(82)90038-1

Induction of Epstein-Barr virus (EBV) in the human lymphoma lines P 3HR-1, and Daudi by TPA (phorbol-12-myristate-13-acetate), n-butyrate, and superinfection with P 3HR-1 virus is associated with an increase in the number of cells bearing Fc receptor (Fc-R). The number of Fc-R per cell, as measured by flow cytometry, is also increased in both cell lines after effective induction of EBV antigens. The increase in cells bearing Fc-R correlates with increased expression of the membrane antigen complex (MA). A high proportion of induced cells concomitantly express both Fc-R and MA in double fluorescence studies. Fc-R activity was not induced by these treatments in EBV-negative lines. After induction of B-95-8 cells (an EBV-transformed marmoset cell line), there is an increase both in MA and whole virus production; however, no Fc-R activity has been detected, suggesting that the B-95-8 EBV strain is incapable of inducing an Fc-R at the cell surface.