Protein expression and subcellular localization of familial acute myelogenous leukemia-related factor

• Published in: Oncology reports Vol. 30; no. 6; pp. 2672 - 2676
• Main Authors: HUANG, YUANMAO, CHEN, LULU, YE, MEILING, LIN, SHENGLONG, ZI, YOUMEI, WANG, SHAOYUAN
• Format: Journal Article
• Language: English
• Published: Greece D.A. Spandidos 01.12.2013; Spandidos Publications UK Ltd
• Subjects: Amino acids; Animals; antibody; Antigens; Blood diseases; Bone marrow; Cloning; DNA methylation; familial acute myeloid leukemia; FAMLF; Gene expression; Gene Expression Regulation, Neoplastic; Humans; Immunization; Kinases; Leukemia; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - etiology; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - genetics; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - pathology; Localization; Medical prognosis; Mice; Mutation; Neoplasm Proteins - biosynthesis; Neoplasm Proteins - genetics; NIH 3T3 Cells; nuclear expression; Patients; Polyclonal antibodies; Polypeptides; Protein expression; Proteins; Proteins - genetics; Proteins - metabolism; Signal transduction; Transcription factors; Tumor Cells, Cultured
• ISSN: 1021-335X; 1791-2431
• DOI: 10.3892/or.2013.2773

The present study was designed to evaluate the expression and subcellular distribution of the familial acute myelogenous leukemia-related factor (FAMLF). A 14-amino acid epitope of the predicted open reading frame of the FAMLF gene was identified using bioinformatics. This polypeptide was synthesized, conjugated to keyhole limpet hemocyanin and was subsequently used to produce antibodies. The antibody titer and specificity were characterized using ELISA and western blot assays, respectively. The antibody detected FAMLF protein expression in several human leukemia cell lines, bone marrow cells derived from one acute myeloid leukemia patient and one chronic myeloid leukemia patient, but not in bone marrow cells of healthy subjects. The FAMLF/GFP fusion protein was expressed in both the nucleus and the cytoplasm of transfected NIH3T3 cells. Our results demonstrate that the FAMLF gene is expressed in an AML patient but not in healthy controls, suggesting its association with AML.