Macrophage mediated tumor cell destruction measured by an alkaline phosphatase assay

A new assay to measure the cytotoxic or growth-inhibitory activity of macrophages against tumor cells is described. The method is based on the fact that, in contrast to macrophages, natural killer cells or cytotoxic lymphocytes, a variety of tumor cells have a very high content of alkaline phosphata...

Full description

Saved in:
Bibliographic Details
Published inJournal of immunological methods Vol. 174; no. 1; pp. 203 - 208
Main Authors Modolell, M., Munder, P.G.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 14.09.1994
Subjects
Alkaline Phosphatase
Animals
Cytotoxic assay
Cytotoxicity, Immunologic
Immunity, Cellular
In Vitro Techniques
Macrophage
Macrophages - immunology
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Tumor Cells, Cultured - immunology
Alkaline phosphatase
ET-18-OCH3
OD
CSF
Macrophage
Cytotoxic assay
Online AccessGet full text

Cover

More Information
Summary:A new assay to measure the cytotoxic or growth-inhibitory activity of macrophages against tumor cells is described. The method is based on the fact that, in contrast to macrophages, natural killer cells or cytotoxic lymphocytes, a variety of tumor cells have a very high content of alkaline phosphatase. The strong linearity between tumor cell number and alkaline phosphatase activity in the cultures permits evaluation of macrophage function with standard ELISA equipment.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:0022-1759
1872-7905
DOI:10.1016/0022-1759(94)90023-X