cDNA Cloning and Sequence Analysis of the Rice Cinnamate-4-Hydroxylase Gene, a Cytochrome P450-Dependent Monooxygenase Involved in the General Phenylpropanoid Pathway

• Published in: Journal of plant biology = Singmul Hakhoe chi Vol. 48; no. 3; pp. 311 - 318
• Main Authors: Yang, D.H. (Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea), Chung, B.Y. (Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea), E-mail: bychung@kaeri.re.kr, Kim, J.S. (Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea), Kim, J.H. (Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea), Yun, P.Y. (Chungnam National University, Daejeon, Republic of Korea), Lee, Y.K. (Chungnam National University, Daejeon, Republic of Korea), Lim, Y.P. (Chungnam National University, Daejeon, Republic of Korea), Lee, M.C. (National Institute of Agricultural Biotechnology, RDA, Suwon, Republic of Korea)
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer Nature B.V 01.09.2005
• Subjects: Amino acid sequence; Amino acids; ARROZ; Biosynthesis; C4H; Cinnamate 4-hydroxylase; Cloning; Cytochrome; Cytochrome P450; Cytochromes P450; Gene expression; Lipids; Nucleotide sequence; Oryza sativa japonica; OsC4HL; Phenylpropanoids; Phylogeny; Regulatory sequences; RICE; RIZ; Sequence analysis; Sorghum bicolor; Terpenes; Wounding
• ISSN: 1226-9239; 1867-0725
• DOI: 10.1007/bf03030528

Plant cytochrome P450 monooxygenases (P450s) mediate a wide range of oxidative reactions involved in the biosynthesis of phenylpropanoids, terpenes, lipids, and alkaloids. We isolated a cDNA clone for cinnamate-4-hydroxylase (C4H) from a Japonica type rice (Oryza sativa L. cv. Ilpumbyeo). This C4H has a deduced amino acid sequence that is 85% identical to that of Sorghum bicolor. Our phylogenetic analysis also showed that the OsC4HL gene is closely related to C4H from S. biocolor.