MicroRNA-22 and microRNA-140 suppress NF-κB activity by regulating the expression of NF-κB coactivators

• Published in: Biochemical and biophysical research communications Vol. 411; no. 4; pp. 826 - 831
• Main Authors: Takata, Akemi, Otsuka, Motoyuki, Kojima, Kentaro, Yoshikawa, Takeshi, Kishikawa, Takahiro, Yoshida, Haruhiko, Koike, Kazuhiko
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 12.08.2011
• Subjects: Adaptor Proteins, Signal Transducing - genetics; Base Sequence; Cell Line; Gene Expression Regulation; Genes, Reporter; Hepatocytes; Hepatocytes - metabolism; Humans; Liver - metabolism; Luciferases - genetics; MicroRNA; MicroRNAs - genetics; MicroRNAs - metabolism; NCOA1; NF-kappa B - antagonists & inhibitors; NF-kappa B - metabolism; NF-κB; NRIP1; Nuclear Proteins - genetics; Nuclear Receptor Coactivator 1 - genetics; Promoter Regions, Genetic; NCOA1; NF-κB; MicroRNA; NRIP1
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2011.07.048

► miRNAs were screened for their ability to regulate NF-κB activity. ► miRNA-22 and miRNA-140-3p suppress NF-κB activity by regulating coactivators. ► miRNA-22 targets nuclear receptor coactivator 1 (NCOA1). ► miRNA-140-3p targets nuclear receptor-interacting protein 1 (NRIP1). Nuclear factor κB (NF-κB) is a transcription factor that regulates a set of genes that are critical to many biological phenomena, including liver tumorigenesis. To identify microRNAs (miRNAs) that regulate NF-κB activity in the liver, we screened 60 miRNAs expressed in hepatocytes for their ability to modulate NF-κB activity. We found that miRNA-22 and miRNA-140-3p significantly suppressed NF-κB activity by regulating the expression of nuclear receptor coactivator 1 (NCOA1) and nuclear receptor-interacting protein 1 (NRIP1), both of which are NF-κB coactivators. Our results provide new information about the roles of miRNAs in the regulation of NF-κB activity.