Diminished adhesion of endothelial aortic cells on fibronectin and collagen layers after nonenzymatic glycation

Adhesion of bovine endothelial cells on fibronectin and collagen before and after nonenzymatic glycation in vitro has been studied. Nonenzymatic glycation of these proteins reduced their ability to bind endothelial cells. Furthermore, nonenzymatically glycated fibronectin failed to bind to normal an...

Full description

Saved in:
Bibliographic Details
Published inExperimental and clinical endocrinology Vol. 91; no. 2; p. 155
Main Authors Krantz, S, Lober, M, Thiele, M, Teuscher, E
Format Journal Article
LanguageEnglish
Published Germany 01.05.1988
Subjects
Animals
Cattle
Collagen - metabolism
Collagen - physiology
Endothelium, Vascular - cytology
Endothelium, Vascular - physiology
Fibronectins - metabolism
Fibronectins - physiology
Gelatin - metabolism
Gelatin - physiology
Glucose - metabolism
Online AccessGet more information

Cover

More Information
Summary:Adhesion of bovine endothelial cells on fibronectin and collagen before and after nonenzymatic glycation in vitro has been studied. Nonenzymatic glycation of these proteins reduced their ability to bind endothelial cells. Furthermore, nonenzymatically glycated fibronectin failed to bind to normal and nonenzymatically glycated gelatin and to fibrin. So gelatin and fibrin Sepharoses can be used to separate highly glycated fibronectins from fibronectins with a low degree of nonenzymatic glucose substitution. Sodium dodecylsulfate polyacrylamide gel electrophoresis did not demonstrate a covalent cross-link between nonenzymatically glycated fibronectins. These results present further evidences for the role of nonenzymatic glycation of proteins in the development of vascular complications in long-term diabetes and of atherosclerosis.
ISSN:0232-7384
DOI:10.1055/s-0029-1210738