Altered gene expressions in rat hippocampus after kainate injection with or without melatonin pre-treatment

• Published in: Neuroscience letters Vol. 359; no. 1; pp. 65 - 68
• Main Authors: Yalcin, Ayfer, Kanit, Lütfiye, Sozmen, Eser Y.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 08.04.2004; Elsevier
• Subjects: Animals; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation - drug effects; Gene Expression Regulation - physiology; Hippocampus; Hippocampus - drug effects; Hippocampus - metabolism; Kainic acid; Kainic Acid - pharmacology; Male; Melatonin; Melatonin - pharmacology; mRNA; Proto-Oncogene Proteins c-bcl-2 - biosynthesis; Proto-Oncogene Proteins c-bcl-2 - genetics; Rats; Rats, Sprague-Dawley; Real-time polymerase chain reaction; RNA, Messenger - biosynthesis; RNA, Messenger - genetics; Vertebrates: nervous system and sense organs; Melatonin; mRNA; Kainic acid; Hippocampus; Real-time polymerase chain reaction; Rat; Rodentia; Central nervous system; Gene expression; Encephalon; Polymerase chain reaction; Vertebrata; Mammalia; Animal; Pineal hormone
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/j.neulet.2004.02.013

The expressions of Bcl-2, Bax and thioredoxin (Trx) mRNAs after kainic acid (KA) injection with or without melatonin pre-treatment were examined by real-time quantitative reverse transcription polymerase chain reaction in rat hippocampus. Bcl-2, Bax, and Trx mRNA expressions after KA injection were significantly increased. Additionally, it was observed that melatonin or melatonin pre-treatment had no significant effect on the regulation of Trx mRNA. Pre-treatment with melatonin at the 30th minute before KA injection resulted in a significant depletion in Bcl-2, Bax and Trx mRNA expressions. However, our results showed that melatonin pre-treatment increases the ratio of Bcl-2 to Bax mRNA in short-term period.