Lowry protein determination on membrane preparations: Need for standardization by amino acid analysis

The protein content of three membrane protein preparations has been determined by the Lowry method with bovine serum albumin as a standard and also by quantitative amino acid analysis as an absolute method. The results differ considerably, the Lowry method giving 29–42% higher values. This implies t...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 124; no. 2; pp. 349 - 352
Main Authors Peters, W.H.M., Fleuren-Jakobs, A.M.M., Kamps, K.M.P., de Pont, J.J.H.H.M., Bonting, S.L.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.01.1982
Subjects
Adenosine Triphosphatases - analysis
Amino Acids - analysis
Animals
Cell Membrane - enzymology
Colorimetry - methods
Gastric Mucosa - enzymology
H(+)-K(+)-Exchanging ATPase
Indicators and Reagents
Kidney Medulla - enzymology
Lowry assay
membrane proteins
Proteins - analysis
Rabbits
Serum Albumin, Bovine - analysis
Sodium-Potassium-Exchanging ATPase - analysis
Swine
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Summary:The protein content of three membrane protein preparations has been determined by the Lowry method with bovine serum albumin as a standard and also by quantitative amino acid analysis as an absolute method. The results differ considerably, the Lowry method giving 29–42% higher values. This implies that many published data for such proteins, based on Lowry protein determinations with bovine serum albumin as the generally applied standard, are in error. Suggestions are made on how to standardize the Lowry method so that reliable values can be obtained for membrane protein.
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ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(82)90050-1