Visible light mutagenesis in Escherichia coli

Visible light (450 nm) caused base-pair substitution and frameshift mutations in Escherichia coli. Using the defined trpA reversion system, we showed that visible light induced transversions at both A:T and G:C sites although some base-pair substitutions were refractory to light-induced mutagenesis,...

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Bibliographic Details
Published inMutation research Vol. 95; no. 2-3; p. 171
Main Authors McGinty, L D, Fowler, R G
Format Journal Article
LanguageEnglish
Published Netherlands 01.01.1982
Subjects
Alleles
Escherichia coli - genetics
Escherichia coli - radiation effects
Genetic Code
Light
Mutation
Plasmids
Transcription, Genetic
Tryptophan - genetics
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Summary:Visible light (450 nm) caused base-pair substitution and frameshift mutations in Escherichia coli. Using the defined trpA reversion system, we showed that visible light induced transversions at both A:T and G:C sites although some base-pair substitutions were refractory to light-induced mutagenesis, possibly because of their location. The presence of R plasmid pKM101 caused a greater than additive increase in light-induced base-pair substitutions for some trpA alleles. Visible light mutagenesis was reduced but not abolished in a recA56 background, suggesting that more than one mutational mechanism may be operative.
ISSN:0027-5107
DOI:10.1016/0027-5107(82)90255-X