Visible light mutagenesis in Escherichia coli
Visible light (450 nm) caused base-pair substitution and frameshift mutations in Escherichia coli. Using the defined trpA reversion system, we showed that visible light induced transversions at both A:T and G:C sites although some base-pair substitutions were refractory to light-induced mutagenesis,...
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Published in | Mutation research Vol. 95; no. 2-3; p. 171 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Netherlands
01.01.1982
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Subjects | |
Online Access | Get more information |
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Summary: | Visible light (450 nm) caused base-pair substitution and frameshift mutations in Escherichia coli. Using the defined trpA reversion system, we showed that visible light induced transversions at both A:T and G:C sites although some base-pair substitutions were refractory to light-induced mutagenesis, possibly because of their location. The presence of R plasmid pKM101 caused a greater than additive increase in light-induced base-pair substitutions for some trpA alleles. Visible light mutagenesis was reduced but not abolished in a recA56 background, suggesting that more than one mutational mechanism may be operative. |
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ISSN: | 0027-5107 |
DOI: | 10.1016/0027-5107(82)90255-X |