Metabolism of exogenous uridine 5'-triphosphate, adenosine 5'-triphosphate and pyrophosphate by alkylsulfatase-producing bacteria

1. 1. The uptake and metabolism of β-P 32-labelled UTP, ATP and uniformly labelled PPi was studied using bacteria washed twice with and immediately resuspended in a medium containing 0.031 M PO 3− 4. 2. 2. Under these conditions, little P 32-labelled PO 3− 4 was detected extracellularly when the cel...

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Bibliographic Details
Published inInternational journal of biochemistry Vol. 13; no. 9; pp. 1019 - 1026
Main Authors Stewart, Gregory J., Fitzgerald, John W.
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 1981
Subjects
Adenosine Triphosphate - metabolism
Diphosphates - metabolism
Enzyme Induction - drug effects
Hydrolysis
Magnesium - pharmacology
Magnesium Chloride
Phosphates - metabolism
Pseudomonas - metabolism
Pseudomonas aeruginosa
Pseudomonas aeruginosa - metabolism
Sulfatases - biosynthesis
Uracil Nucleotides - metabolism
Uridine Triphosphate - metabolism
Uridine Triphosphate - pharmacology
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Summary:1. 1. The uptake and metabolism of β-P 32-labelled UTP, ATP and uniformly labelled PPi was studied using bacteria washed twice with and immediately resuspended in a medium containing 0.031 M PO 3− 4. 2. 2. Under these conditions, little P 32-labelled PO 3− 4 was detected extracellularly when the cells were exposed for 2–5 min to 5 × 10 −5 M concentrations of the above effectors. 3. 3. Cells of both Pseudomonas aeruginosa and Pseudomonas C 12B incorporated the P 32-label from all effectors under the above conditions.
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ISSN:0020-711X
DOI:10.1016/0020-711X(81)90008-2