Colorimetric determination of macrolide antibiotics using ferric ion

Macrolide antibiotics such as erythromycin, oleandomycin, spiromycin, and tylosin are found to react with Fe 3+ in the presence of an acetic acid-sulfuric acid mixture to form a colored product having a useful absorption band at 592 nm. Troleandomycin forms only a weakly colored product upon reactio...

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Bibliographic Details
Published inTalanta (Oxford) Vol. 42; no. 10; pp. 1425 - 1432
Main Authors Gallagher, Patricia A., Danielson, Neil D.
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 01.10.1995
Oxford Elsevier
Subjects
Analysis
Biological and medical sciences
General pharmacology
Medical sciences
Pharmacology. Drug treatments
Chemical analysis
Antibiotics
Iron III Ions
Chemical reactions
Colorimetry
Erythromycin
Tylosin
Antibacterial agent
Macrolide
Acid medium
Quantitative analysis
Ultraviolet visible spectrometry
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Summary:Macrolide antibiotics such as erythromycin, oleandomycin, spiromycin, and tylosin are found to react with Fe 3+ in the presence of an acetic acid-sulfuric acid mixture to form a colored product having a useful absorption band at 592 nm. Troleandomycin forms only a weakly colored product upon reaction. The molar absorptivity is about 2900 1 mol −1 cm −1 for erythromycin and the detection limit is 5 μg ml −1. This colorimetric method permits the analysis of fermentation broths containing either erythromycin or tylosin without a separation step.
Bibliography:ObjectType-Article-1
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ISSN:0039-9140
1873-3573
DOI:10.1016/0039-9140(95)98348-J