Selection and Optimization of Medium Components for the Efficient Production of L-Asparaginase by Leucosporidium scottii L115—A Psychrotolerant Yeast

• Published in: Fermentation (Basel) Vol. 8; no. 8; p. 398
• Main Authors: Moguel, Ignacio S., Yamakawa, Celina K., Brumano, Larissa P., Pessoa, Adalberto, Mussatto, Solange I.
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.08.2022
• Subjects: Acute lymphoblastic leukemia; Asparaginase; Bacteria; Carbon; Carbon sources; Cold; Design of experiments; E coli; Enzymes; Glycerol; L-asparaginase; Leucosporidium scottii; Leukemia; Mathematical models; medium composition; Microorganisms; Nitrogen; optimization; Potassium chloride; psychrotolerant; Salts; Statistical analysis; Sucrose; Yeast; Zinc sulfate
• ISSN: 2311-5637; 2311-5637
• DOI: 10.3390/fermentation8080398

This study reports the production of L-asparaginase (ASNase), an enzyme mainly used for the treatment of acute lymphoblastic leukemia, by Leucosporidiumscottii L115, a psychrotolerant yeast isolated from the Antarctic ecosystem. Focus was given to select the most appropriate medium components able to maximize the enzyme production by this yeast, as a first step for the development of a new process to produce ASNase. By combining knowledge in bioprocesses, statistical analysis and modeling, the medium composition that most favored enzyme production was established, which consisted of using a mixture of sucrose (28.34 g L−1) and glycerol (15.61 g L−1) as carbon sources, supplemented with proline (6.15 g L−1) and the following salts (g L−1): KCl, 0.52; MgSO4·7H2O, 0.52; CuNO3·3H2O, 0.001; ZnSO4·7H2O, 0.001; and FeSO4·7H2O, 0.001. By using this medium, enzyme production of 2850 U L−1 (productivity of 23.75 U L−1 h−1) was obtained, which represented a 28-fold increase in enzyme production per gram of cells (178 U gdcw−1) when compared to the control (non-optimized medium), and a 50-fold increase when compared to a reference medium used for ASNase production.