Choosing an equivalent replacement column for a reversed-phase liquid chromatographic assay procedure

• Published in: Journal of Chromatography A Vol. 1057; no. 1; pp. 59 - 74
• Main Authors: Dolan, J.W., Maule, A., Bingley, D., Wrisley, L., Chan, C.C., Angod, M., Lunte, C., Krisko, R., Winston, J.M., Homeier, B.A., McCalley, D.V., Snyder, L.R.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 19.11.2004; Elsevier
• Subjects: Analytical chemistry; Cation-exchange capacity; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, Liquid - instrumentation; Column selectivity; Exact sciences and technology; Hydrogen Bonding; Hydrogen-bond acidity; Hydrogen-bond basicity; Hydrophobicity; Other chromatographic methods; Pharmaceutical Preparations - analysis; Selectivity parameters; Sensitivity and Specificity; Steric resistance; Hydrogen-bond acidity; Column selectivity; Hydrophobicity; Selectivity parameters; Steric resistance; Hydrogen-bond basicity; Cation-exchange capacity; Exchange capacity; Cation exchange; Lipophily; Acidity; Liquid chromatography; Selectivity; Reversed phase chromatography; Bonded stationary phase; Physicochemical properties; Hydrogen bond
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2004.09.064

The column selectivity parameters ( H, S *, A, B and C) described in the preceding paper [L.R. Snyder, A. Maule, A. Heebsch, R. Cuellar, S. Paulson, J. Carrano, L. Wrisley C.C. Chan, N. Pearson, J.W. Dolan, J.J. Gilroy, J. Chromatogr. A 1057 (2004) 49–57] can be used to compare columns in terms of selectivity. A detailed procedure for such column comparisons is presented here, and evaluated by its use in finding suitable replacement columns for 12 different routine separations performed in five different pharmaceutical analysis laboratories.