Photomodification of RNA and DNA fragments by oligonucleotide reagents bearing arylazide groups

• Published in: Biochimie Vol. 75; no. 1; pp. 25 - 27
• Main Authors: Levina, A.S., Berezovskii, M.V., Venjaminova, A.G., Dobrikov, M.I., Repkova, M.N., Zarytova, V.F.
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 1993
• Subjects: arylazide derivatives of oligonucleotides; Azides - chemistry; DNA - chemistry; Indicators and Reagents; Oligonucleotides - chemistry; Photochemistry; Photomodification of RNA- and DNA-fragments; RNA - chemistry; sN, 2-nitro-5-azidobenzamido group; arylazide derivatives of oligonucleotides; Photomodification of RNA- and DNA-fragments; p-azidotetrafluorobenzamido group
• ISSN: 0300-9084; 1638-6183
• DOI: 10.1016/0300-9084(93)90020-S

Photomodification of ribo- and deoxyribo-octanucleotides by oligonucleotide reagents (6- and 7-mers) bearing p-azidotetraflurobenzamido and 2-nitro-5-azidobenzamido groups has been investigated. It is shown that the oligonucleotides with a perfluoroarylazide group were effective modifiers both of deoxyribo- and ribo-targets. Maximum extent of cross-linked product formation (70%) was obtained when the deoxyribo-octanucleotide was modified by a heptanucleotide reagent with a perfluoroarylazide group. Selectively of the photomodification was also high (50% on the G-residue at a certain position).