Photomodification of RNA and DNA fragments by oligonucleotide reagents bearing arylazide groups
Photomodification of ribo- and deoxyribo-octanucleotides by oligonucleotide reagents (6- and 7-mers) bearing p-azidotetraflurobenzamido and 2-nitro-5-azidobenzamido groups has been investigated. It is shown that the oligonucleotides with a perfluoroarylazide group were effective modifiers both of de...
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Published in | Biochimie Vol. 75; no. 1; pp. 25 - 27 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
France
Elsevier Masson SAS
1993
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Subjects | |
Online Access | Get full text |
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Summary: | Photomodification of ribo- and deoxyribo-octanucleotides by oligonucleotide reagents (6- and 7-mers) bearing
p-azidotetraflurobenzamido and 2-nitro-5-azidobenzamido groups has been investigated. It is shown that the oligonucleotides with a perfluoroarylazide group were effective modifiers both of deoxyribo- and ribo-targets. Maximum extent of cross-linked product formation (70%) was obtained when the deoxyribo-octanucleotide was modified by a heptanucleotide reagent with a perfluoroarylazide group. Selectively of the photomodification was also high (50% on the G-residue at a certain position). |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0300-9084 1638-6183 |
DOI: | 10.1016/0300-9084(93)90020-S |