Pcr-based method for identifying the S-genotypes of Japanese pear cultivars

• Published in: Theoretical and applied genetics Vol. 98; no. 6-7; pp. 961 - 967
• Main Authors: ISHIMIZU, T, INOUE, K, SHIMONAKA, M, SAITO, T, TERAI, O, NORIOKA, S
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.05.1999; Berlin Springer Nature B.V
• Subjects: Biological and medical sciences; Classical genetics, quantitative genetics, hybrids; Cultivars; Enzymes; Experiments; Fisheries; Fruits; Fundamental and applied biological sciences. Psychology; Genetic aspects; Genetics of eukaryotes. Biological and molecular evolution; Genotype; Genotype & phenotype; Horticulture; Identification and classification; Methods; Pear; Plant genetics; Plant reproduction; Plants; Polymerase chain reaction; Pteridophyta, spermatophyta; Pyrus pyrifolia; Rosaceae; Self-incompatibility; Vegetals; Asia; Japan; Polymerase chain reaction; Selfincompatibility; Analysis method; Dicotyledones; Angiospermae; Rosaceae; Genotype; Spermatophyta; Fruit tree; Cultivar
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s001220051156

Japanese pear (Pyrus pyrifolia Nakai), a member of the Rosaceae, shows gametophytic self-incompatibility that is controlled by the S-locus. The S-genotype of Japanese pear cultivars is an important factor for crossing and breeding. We report a rapid reliable method to identify these S-genotypes. It consists of PCR amplification of the S-RNase gene from genomic DNA and subsequent digestion of the PCR fragments with S-allele-specific restriction endonucleases. Using this method, we determined the unknown S-genotypes of nine Japanese pear cultivars and selected self-compatible varieties from the offspring of the self-compatible cultivar, 'Osa-Nijisseiki'.