Phospholipid changes in the bones of the vitamin D-deficient, phosphate-deficient, immature rat

• Published in: Metabolic bone disease & related research Vol. 5; no. 2; pp. 81 - 85
• Main Authors: Boskey, A.L., Timchak, D.M.
• Format: Journal Article
• Language: English
• Published: France Elsevier Inc 1983
• Subjects: Acidic Phospholipids; Animals; Bone and Bones - analysis; Bone and Bones - metabolism; Bone Lipids; Ca-Acidic Phospholipid; Epiphyses - analysis; Epiphyses - metabolism; Female; Male; Mineralization; Phosphate Complexes; Phosphates - deficiency; Phospholipids - metabolism; Rats; Rats, Inbred Strains; Rickets; Vitamin D; Vitamin D Deficiency - metabolism; Bone Lipids; Rickets; Vitamin D; Phosphate Complexes; Mineralization; Ca-Acidic Phospholipid; Acidic Phospholipids
• ISSN: 0221-8747; 1878-061X
• DOI: 10.1016/0221-8747(83)90006-1

The epiphyseal (cartilage) and diaphyseal (bone) regions of the long bones of vitamin D-deficient, phosphate-deficient, immature rats have been shown to contain CaPLPO 4 complexes in amounts comparable to that found in normal rat bones. This suggests that these calcium acidic phospholipid complexes are formed prior to mineralization. The metaphysis (bone and calcified cartilage) of the experimental animals contained less CaPLPO 4 than control bone, which suggests that CaPLPO 4 content is elevated as mineralizing activity increases. Overall bone lipid composition was dependent on the vitamin D status of the animals. Total lipid, cholesterol, and cholesterol ester content was higher in experimental animals than in controls. In contrast, free fatty acid and lysophosphatide were lower in experimental than in control animal's bones. The total phospholipid content (based on organic phosphate analyses) was unaltered by the vitamin D status. These lipid changes, reminiscentof changes seen in the intestinal brush border membrane of rachitic animals, suggest that vitamin D effects on lipid metabolism in bone may be similar to those in the intestine. Il a été montré qua lea zones épiphysaires (contenant du cartilage) et diaphysaires (contenent de I'os) d'os longs de jeunes rats carencés on vitamins D et on phosphate contenatent la même quentité de complexes calcium-phospholipides-phosphate (CaPLPO 4) quo cones de rats normaux. Cola suggére que ces complexes sont formés antérieurement à la minéralisation. Mats la métaphyse (contenant de I'os et du cartilage calcitlé) des animaux en expérimentation contient moans de complexes CaPL1304 qua cells des os témoins, ce qua suggére qua Is contenu an Ca-PL-1304 s'accrolt an même temps quo l'activlté de minéralisatton. La teneur totals on Itpides de l'os depend du degré de carence an vitamins D des animaux : Is contenu an lipides totaux on cholestérol et an cholestérol estérfté set plus élevé chez lea animaux an expérimentation qua chez lea temoins. Au contralre, lea acides gras libres et lea lyso-phosphatides de l'os sont à un toux plus bas chez lea animaux an expérimentation qua chez lea animaux témoins. Le contenu total an phospholipides- base sur l'evaluation du phosphors organique — West pas modiflé par la carence an vitamins D. Ces modiftcations NpIdlques rappelent celles natées dans la membrane de la bordure on brosse des cellules intestinales ch ez lee animaux rachitiques. Cola suggére qua lea effects de la vitamins D sur la métaboliame des lipides de l'os pourralent être Identiques à ceux notés au niveou de l'intestin.