Fluorogenic Tetrazine-Siliconrhodamine Probe for the Labeling of Noncanonical Amino Acid Tagged Proteins
Tetrazine-bearing fluorescent labels enable site-specific tagging of proteins that are genetically manipulated with dienophile modified noncanonical amino acids. The inverse electron demand Diels-Alder reaction between the tetrazine and the dienophile fulfills the criteria of bioorthogonality allowi...
Saved in:
Published in | Methods in molecular biology (Clifton, N.J.) Vol. 1728; p. 337 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
2018
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Summary: | Tetrazine-bearing fluorescent labels enable site-specific tagging of proteins that are genetically manipulated with dienophile modified noncanonical amino acids. The inverse electron demand Diels-Alder reaction between the tetrazine and the dienophile fulfills the criteria of bioorthogonality allowing fluorescent labeling schemes of live cells. Here, we describe the detailed synthetic and labeling protocols of a near infrared emitting siliconrhodamine-tetrazine probe suitable for super-resolution imaging of residue-specifically engineered proteins in mammalian cells. |
---|---|
ISSN: | 1940-6029 |
DOI: | 10.1007/978-1-4939-7574-7_22 |