Preparation of Oil Palm Empty Fruit Bunch Hydrolysate

Malaysia is the second largest palm oil producer and exporter globally. When crude palm oil is produced in both plantations and oil processing mills, a large amount of oil palm empty fruit bunch (OPEFB) is simultaneously produced as a waste product. Here, we describe the preparation of hydrolysate f...

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Bibliographic Details
Published inFermentation (Basel) Vol. 7; no. 2; p. 81
Main Authors Akita, Hironaga, Yusoff, Mohd Zulkhairi Mohd, Fujimoto, Shinji
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.06.2021
Subjects
acid hydrolysis
Acids
Arabinose
Biomass
Carbon
Cellulase
Cellulose
Chromatography
Efficiency
enzymatic hydrolysis
Enzymes
Fermentation
Galactose
Glucose
Hemicellulose
High-performance liquid chromatography
hydrolysate
Hydrolysates
hydrothermal pretreatment
Lignin
Lignocellulose
Mannose
Methods
Microorganisms
oil palm empty fruit bunch
Vegetable oils
United States > US
Japan
Malaysia
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Summary:Malaysia is the second largest palm oil producer and exporter globally. When crude palm oil is produced in both plantations and oil processing mills, a large amount of oil palm empty fruit bunch (OPEFB) is simultaneously produced as a waste product. Here, we describe the preparation of hydrolysate from OPEFB. After OPEFB was hydrothermally treated at 180–200 °C, the resultant liquid phase was subjected to high-performance liquid chromatography analysis, while the solid phase was used for acidic and enzymatic hydrolysis. Hemicellulose yield from the acid-treated solid phase decreased from 153 mg/g-OPEFB to 27.5 mg/g-OPEFB by increasing the hydrothermal treatment temperature from 180 to 200 °C. Glucose yield from the enzyme-treated solid phase obtained after hydrothermal treatment at 200 °C was the highest (234 ± 1.90 mg/g-OPEFB, 61.7% production efficiency). In contrast, xylose, mannose, galactose, and arabinose yields in the hydrolysate prepared from the solid phase hydrothermally treated at 200 °C were the lowest. Thus, we concluded that the optimum temperature for hydrothermal pretreatment was 200 °C, which was caused by the low hemicellulose yield. Based on these results, we have established an effective method for preparing OPEFB hydrolysates with high glucose content.
ISSN:2311-5637
2311-5637
DOI:10.3390/fermentation7020081