Novosphingobium sediminicola sp. nov. isolated from freshwater sediment

• Published in: International journal of systematic and evolutionary microbiology Vol. 61; no. Pt 10; pp. 2464 - 2468
• Main Authors: Baek, Sang-Hoon, Lim, Ju Hyoung, Jin, Long, Lee, Hyung-Gwan, Lee, Sung-Taik
• Format: Journal Article
• Language: English
• Published: Reading International Union of Microbiological Societies 01.10.2011; Society for General Microbiology
• Subjects: Animal, plant and microbial ecology; Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; Cluster Analysis; cytoplasm; DNA; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fresh Water - microbiology; freshwater; Fundamental and applied biological sciences. Psychology; Geologic Sediments - microbiology; Glycosphingolipids - analysis; Microbial ecology; Microbiology; Molecular Sequence Data; new species; nucleotide sequences; Phylogeny; Pigments, Biological - metabolism; Polyamines - analysis; Quinones - analysis; ribosomal RNA; RNA, Ribosomal, 16S - genetics; sediments; Sequence Analysis, DNA; sequence homology; spermidine; Sphingomonadaceae - classification; Sphingomonadaceae - genetics; Sphingomonadaceae - isolation & purification; Sphingomonadaceae - physiology; Sphingomonas; Systematics; Various environments (extraatmospheric space, air, water); Freshwater environment; Bacteria; New species; Sediments; Taxonomy
• ISSN: 1466-5034; 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.024307-0

A yellow-pigmented, Gram-negative, short rod-shaped, non-motile and non-spore-forming bacterial strain, designated HU1-AH51T, was isolated from freshwater sediment and was characterized using a polyphasic approach, in order to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarity, strain HU1-AH51T was shown to belong to the genus Novosphingobium, showing the highest level of sequence similarity with respect to Novosphingobium resinovorum NCIMB 8767T (96.0 %), Novosphingobium naphthalenivorans TUT562T (96.0 %) and Novosphingobium panipatense SM16T (96.0 %). Strain HU1-AH51T had a genomic DNA G+C content of 62.6 mol% and Q-10 as the predominant respiratory quinone. Furthermore, the major polyamine component (spermidine) in the cytoplasm and the presence of sphingoglycolipids suggested that strain HU1-AH51T belongs to the family SPHINGOMONADACEAE: On the basis of its phenotypic properties and phylogenetic distinctiveness, strain HU1-AH51T represents a novel species of the genus Novosphingobium, for which the name Novosphingobium sediminicola sp. nov. is proposed. The type strain is HU1-AH51T ( = LMG 24320T = KCTC 22311T).