A molecular approach in the analysis of the faecal bacterial community in an African millipede belonging to the family Spirostreptidae (Diplopoda)

• Published in: European journal of soil biology Vol. 38; no. 1; pp. 67 - 70
• Main Authors: Oravecz, Orsolya, Nyiro&#x030B, Gábor, Márialigeti, Károly
• Format: Journal Article
• Language: English
• Published: Paris Elsevier Masson SAS 2002; Editions scientifiques et médicales Elsevier
• Subjects: 16S rDNA sequencing; Actinobacteria; Animal, plant and microbial ecology; ARDRA; BACTERIA; BIOLOGIA MOLECULAR; Biological and medical sciences; BIOLOGIE MOLECULAIRE; Cellulomonadaceae; DIPLOPODA; FAECES; FAUNA DEL SUELO; FAUNE DU SOL; FECES; FLORA MICROBIANA; FLORE MICROBIENNE; FOREST SOILS; Fundamental and applied biological sciences. Psychology; HECES; INTESTIN; INTESTINES; INTESTINOS; MAROC; MARRUECOS; Microbial ecology; MICROBIAL FLORA; MOLECULAR BIOLOGY; MOROCCO; Nocardiaceae; Normal microflora of man and animals. Rumen; RHODOCOCCUS (BACTERIA); SOIL FAUNA; SOL DE FORET; SUELO FORESTAL; Africa; ARDRA; Actinobacteria; Nocardiaceae; 16S rDNA sequencing; Diplopoda; Cellulomonadaceae; Animal microorganism relation; Nucleotide sequence; Digestive system; Fauna; Ribosomal DNA; Gut; Rhodococcus; Actinomycetales; Cellulomonas; Macrofauna; Arthropoda; Myriapoda; Bacteria; Actinomycetes; New species; Associated microflora; Feces; Invertebrata; Microbial community
• ISSN: 1164-5563
• DOI: 10.1016/S1164-5563(01)01128-1

Forty selected bacterial isolates of the faecal microbial community in an African millipede belonging to the family Spirostreptidae were characterized by amplified ribosomal DNA-restriction analysis (ARDRA) and by sequencing either the first 500 bp or almost the entire length of the 16S rRNA gene. Sequence data show that the faecal microbial community investigated is dominated by members of the class Actinobacteria with most of the strains belonging to the genera Rhodococcus, Cellulomonas and Leifsonia. In certain cases, low similarity values to the previously described 16S rDNA sequences suggest that these strains presumably constitute new species within the Actinobacteria.