Expression of mRNAs for PACAP and its receptor in human neuroblastomas and their relationship to catecholamine synthesis

• Published in: Regulatory peptides Vol. 123; no. 1-3; pp. 29 - 32
• Main Authors: Isobe, Kazumasa, Kaneko, Michio, Kaneko, Setsuko, Nissato, Sumiko, Nanmoku, Toru, Takekoshi, Kazuhiro, Okuda, Yukichi, Kawakami, Yasushi
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Shannon Elsevier B.V 15.12.2004; Amsterdam Elsevier
• Subjects: Base Sequence; Biological and medical sciences; Catecholamines - biosynthesis; Cell Line, Tumor; Child, Preschool; DNA Primers - genetics; Female; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Neoplastic; Humans; Infant; Male; Medical sciences; Nerve Growth Factors - genetics; Neuroblastoma; Neuroblastoma - genetics; Neuroblastoma - metabolism; Neurology; Neuropeptides - genetics; Neurotransmitter Agents - genetics; Pituitary Adenylate Cyclase-Activating Polypeptide; Pituitary adenylate cyclase-activating polypeptide (PACAP); Receptors, Cell Surface - genetics; Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide; Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I; Receptors, Vasoactive Intestinal Peptide - genetics; Receptors, Vasoactive Intestinal Polypeptide, Type I; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Messenger - metabolism; RNA, Neoplasm - genetics; RNA, Neoplasm - metabolism; Tumors of the nervous system. Phacomatoses; Tyrosine 3-Monooxygenase - genetics; Tyrosine hydroxylase (TH); Vasoactive Intestinal Peptide - metabolism; Vertebrates: endocrinology; Tyrosine hydroxylase (TH); Neuroblastoma; Pituitary adenylate cyclase-activating polypeptide (PACAP); Human; Nervous system diseases; Enzyme; PACAP receptor; Biosynthesis; Malignant tumor; Catecholamine; Gene expression; Tyrosine 3-monooxygenase; Pituitary adenylate cyclase-activating polypeptide (PAC'AP); Neuropeptide; In vitro; Oxidoreductases; Autonomic neuropathy; Pituitary adenylate cyclase activating peptide; Biological receptor
• ISSN: 0167-0115; 1873-1686
• DOI: 10.1016/j.regpep.2004.05.011

Purpose: Pituitary adenylate cyclase-activating polypeptide (PACAP), a member of the secretin/glucagon/vasoactive intestinal peptide family, induces the expression of catecholamine-synthesizing enzymes in adrenal medullary cells. In addition, PACAP and its receptor have been detected in human neuroblastoma tissues and cell lines, though it is not yet known whether PACAP enhances the expression of genes encoding catecholamine-synthesizing enzymes. To address this question, we analyzed PACAP, PACAP receptor and tyrosine hydroxylase (TH) mRNAs in neuroblastomas. Methods: The levels of mRNA for PACAP and vasoactive intestinal peptide (VIP), as well as their receptors and the mRNA for TH were measured by RT-PCR or real-time PCR analysis. Results: VPAC1R mRNA was detected in all of 16 tissues and 3 cell lines that were examined, while VPAC2R mRNA was detected in 5 of 16 (31%) tissue and 2 of 3 cell lines. PAC1R mRNA was detected in 6 out of 16 (38%) tissues and none of 3 cell lines. mRNA expression of PACAP and TH were detected in many tissues (10/16 and 16/16, respectively). However, neither in tissues nor cell lines did PACAP mRNA expression correlate with TH mRNA expression. Conclusion: Our findings suggest that PACAP is not involved in the regulation of expression of TH in neuroblastomas.