Bottom-up/cross-linking mass spectrometry via simplified sample processing on anion-exchange solid-phase extraction spin column

We introduce a simple single-column protein digestion method for low-microgram-level samples containing sodium dodecyl sulfate and Coomassie dye that can be completed within a few hours.

Saved in:
Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 58; no. 6; pp. 775 - 778
Main Authors Takemori, Ayako, Kawashima, Yusuke, Takemori, Nobuaki
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 18.01.2022
Subjects
Anion exchanging
Crosslinking
Mass spectrometry
Sodium dodecyl sulfate
Solid phases
Online AccessGet full text

Cover

More Information
Summary:We introduce a simple single-column protein digestion method for low-microgram-level samples containing sodium dodecyl sulfate and Coomassie dye that can be completed within a few hours.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1359-7345
1364-548X
DOI:10.1039/d1cc05529a