Thermal regulation of FMLP receptors on human neutrophils

• Published in: Journal of leukocyte biology Vol. 45; no. 6; pp. 529 - 537
• Main Authors: Coles, Rosalind B., Ranney, Richard R., Freer, Richard J., Carchman, Richard A.
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Society for Leukocyte Biology 01.06.1989; Federation of American Societies for Experimental Biology
• Subjects: Analysis of the immune response. Humoral and cellular immunity; Biological and medical sciences; Chemotaxis; Chemotaxis, Leukocyte; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Hot Temperature; Humans; Immunobiology; juvenile periodontitis; lysosomal enzyme secretion; Lysosomes - enzymology; N-Formylmethionine Leucyl-Phenylalanine - metabolism; Neutrophils - pathology; Neutrophils - physiopathology; Neutrophils - ultrastructure; Organs and cells involved in the immune response; Periodontitis - metabolism; Periodontitis - physiopathology; PMNs; receptor ligand binding; Receptors, Formyl Peptide; Receptors, Immunologic - metabolism; Enzyme; Secretion; Granulocyte; Lysosome; Thermal sensibility; Chemotaxis; Membrane receptor; Regulation(control); Fixation; Neutrophile; Distribution; Juvenile periodontitis; Affinity
• ISSN: 0741-5400; 1938-3673
• DOI: 10.1002/jlb.45.6.529

Polymorphonuclear leukocytes (PMNs) from subjects diagnosed as having iuvenile periodontitis (JP) have been categorized on the basis of their chemotactic (CTX) response to f‐met‐leu‐phe (FMLP) when assayed concurrently with PMNs from periodon‐tally healthy subjects (HP). When PMNs from JP groups demonstrating depressed CTX were assayed for lysosomal enzyme secretion (LES) in response to FMLP, there were no significant differences with respect to rate or amount. Significant differences were observed between HP and chemotactically depressed JP cells when assessed for FMLP receptor ligand binding at 23°, but not at 4°. Receptor differences observed at 23° in HP cells included an increase in amount of total binding, number of receptors, and available dispiaceable binding sites, compared with the chemotactically depressed JP PMNs, whereas the receptor affinities were similar. These data suggest that differences in FMLP receptor density in JP PMN that are chemotactically depressed may be related to processes that modulate receptor mobility and/or expression.