Increased activity of MMP‐2 in hypertensive obese children is associated with hypoadiponectinemia

• Published in: Obesity (Silver Spring, Md.) Vol. 23; no. 1; pp. 177 - 182
• Main Authors: Belo, Vanessa A., Lacchini, Riccardo, Miranda, Josiane A., Lanna, Carla M.M., Souza‐Costa, Debora C., Tanus‐Santos, Jose E.
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.01.2015
• Subjects: Adiponectin - deficiency; Adiponectin - metabolism; Adolescent; Atherosclerosis; Bands; Bioavailability; Blood pressure; Case-Control Studies; Child; Cross-Sectional Studies; Diabetes; Endocrinology; Enzyme-Linked Immunosorbent Assay; Enzymes; Female; Humans; Hypertension; Hypertension - complications; Hypertension - metabolism; Insulin; Insulin resistance; Male; Matrix Metalloproteinase 2 - blood; Matrix Metalloproteinase 2 - metabolism; Metabolism, Inborn Errors - complications; Metabolism, Inborn Errors - metabolism; Obesity; Pathogenesis; Pediatric Obesity - complications; Pediatric Obesity - metabolism; Ratios; Regression Analysis; Teenagers; Tissue Inhibitor of Metalloproteinase-2 - blood; Brazil
• ISSN: 1930-7381; 1930-739X; 1930-739X
• DOI: 10.1002/oby.20939

Objective To compare the circulating levels of MMP‐2 and TIMP‐2 and the MMP‐2/TIMP‐2 ratio in control, obese, and obese hypertensive children and adolescents and to assess whether hypoadiponectinemia is associated with MMP‐2 and TIMP‐2 levels and MMP‐2/TIMP‐2 ratios. Methods Studies were carried out with 53 control, 73 obese, and 29 obese hypertensive children and adolescents in this cross‐sectional study. Adiponectin and TIMP‐2 concentrations were measured by ELISA. MMP‐2 concentrations were measured by gelatin zymography. Multiple linear regression analysis was carried out to assess the effects of adiponectin on MMP‐2 and TIMP‐2 levels and MMP‐2/TIMP‐2 ratios. Results The obese hypertensive group had the lowest adiponectin levels among groups (P < 0.05) while obese subjects had lower adiponectin levels than control subjects (P < 0.05). Obese hypertensive subjects also had higher circulating MMP‐2 concentrations than obese subjects (P < 0.05) and had the highest MMP‐2/TIMP‐2 ratios among groups (P < 0.05). Moreover, obese/hypertensive subjects had the lowest TIMP‐2 levels among groups (P < 0.05). A multiple linear regression analysis showed that MMP‐2 levels and MMP‐2/TIMP‐2 ratios are negatively associated with adiponectin levels (P = 0.034 and P = 0.011, respectively) while TIMP‐2 levels is positively associated with adiponectin levels (P = 0.013). Conclusions Increased activity of MMP‐2 (MMP‐2/TIMP‐2 ratio) and reduced TIMP‐2 levels may play an important role in clinical hypertension of childhood obesity. Additionally, hypoadiponectinemia may contribute to increased activity of MMP‐2 in obese/hypertensive children and adolescents.