Observations with the electron microscope on the absorption of India ink from the peritoneal cavity of the rabbit

• Published in: Okajimas Folia Anatomica Japonica Vol. 38; no. 3-4; pp. 149 - 173
• Main Authors: Kotani, Masahiko, Rai, Masao, Nakao, Shiro
• Format: Journal Article
• Language: English
• Published: Japan Editorial Board of Okajimas Folia Anatomica Japonica 1962
• Subjects: Animals; Carbon; Electrons; Ink; Lymphatic System; Microscopy; Microscopy, Electron; Old Medline; Peritoneal Cavity; Rabbits; MICROSCOPY, ELECTRON; PERITONEAL CAVITY; LYMPHATIC SYSTEM; INK
• ISSN: 0030-154X; 1881-1736
• DOI: 10.2535/ofaj1936.38.3-4_149

1) The India ink particles, introduced into the peritoneal cavity, entered the lymphatic capillaries in the tendon parts of the diaphragm through the submesothelial tissue spaces between the collagenous bundles. Frequently, thin cytoplasmic processes of the lymphatic endothelial cells extended on the surface of the submesothelial collagenous bundles to come in direct contact with the peritoneal cells. 2) The India ink particles were carried from the peritoneal cavity through a dilated, canal-like intercellular space between two neighbouring peritoneal cells, or through a opening between two widely separated peritoneal cells into the submesothelial tissue space. The India ink particles were also transferred through the cytoplasm of the peritoneal cells. In the cytoplasm, the carbon granules was situated in the vacuoles, varying in size and shape, and in the opaque bodies. The vacuoles including the carbon granules were greatly increased in number at 60 minutes after injection, although the intercellular spaces were the main pathway at 15 to 30 minutes after injection. 3) From the submesothelial tissue space the carbon particles were transported through the intercellular spaces between the lymphatic endothelial cells, or through the vacuoles in the cytoplasm of the lymphatic endothelial cells into the lumen of the lymphatic capillaries.