MCTR1 alleviates lipopolysaccharide‐induced acute lung injury by protecting lung endothelial glycocalyx

• Published in: Journal of cellular physiology Vol. 235; no. 10; pp. 7283 - 7294
• Main Authors: Li, Hui, Hao, Yu, Yang, Li‐Li, Wang, Xin‐Yang, Li, Xin‐Yu, Bhandari, Suwas, Han, Jun, Liu, Yong‐Jian, Gong, Yu‐Qiang, Scott, Aaron, Smith, Fang Gao, Jin, Sheng‐Wei
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.10.2020
• Subjects: Acute Lung Injury - blood; Acute Lung Injury - chemically induced; Acute Lung Injury - drug therapy; Adaptor Proteins, Signal Transducing - metabolism; Animals; Biodegradation; Cell culture; Cytokines; Cytokines - blood; Degradation; Docosahexaenoic Acids - pharmacology; Endothelial cells; endothelial glycocalyx; Endothelium - drug effects; Endothelium - pathology; Glucuronidase - metabolism; Glycocalyx - drug effects; Glycocalyx - pathology; Heparan sulfate; HPA; Human Umbilical Vein Endothelial Cells; Humans; Inflammation; Inflammation Mediators - blood; Inflammatory response; Interleukins; Lipids; Lipopolysaccharides; Lipopolysaccharides - toxicity; Lung - drug effects; Lung - physiology; Lung - physiopathology; Lungs; Macrophages; Male; MCTR1; Mice; Mice, Inbred C57BL; Permeability; Phagocytosis; Phosphorylation; Regeneration; Sepsis; Sepsis - drug therapy; Sepsis - pathology; Sepsis - physiopathology; Serum levels; Signal Transduction; SIRT1; SIRT1 protein; Sirtuin 1 - metabolism; Syndecan; Tissue engineering; Transcription Factor RelA - metabolism; Umbilical vein; HPA; endothelial glycocalyx; MCTR1; SIRT1; sepsis
• ISSN: 0021-9541; 1097-4652
• DOI: 10.1002/jcp.29628

Endothelial glycocalyx degradation, critical for increased pulmonary vascular permeability, is thought to facilitate the development of sepsis into the multiple organ failure. Maresin conjugates in tissue regeneration 1 (MCTR1), a macrophage‐derived lipid mediator, which exhibits potentially beneficial effects via the regulation of bacterial phagocytosis, promotion of inflammation resolution, and regeneration of tissue. In this study, we show that MCTR1 (100 ng/mouse) enhances the survival of mice with lipopolysaccharide (LPS)‐induced (15 mg/kg) sepsis. MCTR1 alleviates LPS (10 mg/kg)‐induced lung dysfunction and lung tissue inflammatory response by decreasing inflammatory cytokines (tumor necrosis factor‐α, interleukin‐1β [IL‐1β], and IL‐6) expression in serum and reducing the serum levels of heparan sulfate (HS) and syndecan‐1. In human umbilical vein endothelial cells (HUVECs) experiments, MCTR1 (100 nM) was added to the culture medium with LPS for 6 hr. MCTR1 treatment markedly inhibited HS degradation by downregulating heparanase (HPA) protein expression in vivo and in vitro. Further analyses indicated that MCTR1 upregulates sirtuin 1 (SIRT1) expression and decreases NF‐κB p65 phosphorylation. In the presence of BOC‐2 or EX527, the above effects of MCTR1 were abolished. These results suggest that MCTR1 protects against LPS‐induced sepsis in mice by attenuating pulmonary endothelial glycocalyx injury via the ALX/SIRT1/NF‐κB/HPA pathway. Maresin conjugates in tissue regeneration 1 (MCTR1) protects against lipopolysaccharide (LPS)‐induced sepsis in mice by attenuating pulmonary endothelial glycocalyx injury via the ALX/SIRT1/NF‐κB/HPA pathway.